When using a fluorescence microscope, chemicals that emit fluorescence are combined with the light microscope’s magnification abilities. A high-intensity light source is used in fluorescence microscopy to excite a fluorophore, a fluorescent molecule, in the sample being examined.
Bandpass, shortpass, and longpass emission filters, as well as dichroic beamsplitters, are all included in the fluorescence filter sets that GF Tech offers. A range of wavelengths are selected to match the excitation and cut-off wavelengths of the fluorescent dye. Standard substrates are covered with all-dielectric low absorbing hard coatings to create a quick transition between high transmission and deep blocking down to OD5 or OD6. The high transmission of pass band, steef slope and precise cut off of excitation filter and precise cut on of emission filter ensure high quality images in terms of sharpness, contrast and brightness.